Biomeditsina va amaliyot jurnali, 2023 №1

Subject of the article

STUDY OF CHANGES IN THE MICROBIOLOGICAL LANSCAPE OF THE COLON UNDER THE INFLUENCE OF A GENE-MODIFIED PRODUCTS (66-73)

Authors

Karimova A. Maqsuda, Ibrahimova R. Нamida, Asatova B. Nafisa

Institution

Urgench Branch of Tashkent Medical Academy

Abstract

Objective: The study of the effect of GM-soi on the normal microflora of the colon in experimental animals. Methods: To achieve the set goal do this, 90 toothless male feathers were involved in the study, which were divided into 3 groups: group 1 - intact white toothless feathers (n = 30) in the standard vivarium diet, not containing soybeans without GM or GM; Group 2 - white rats (n = 30) comprising soy without GM in the standard vivarium diet; Group 3 - white rats (n = 30) coated with GM soy in the standard vivarium diet. Bacteriological researches of intestinal mass of proteinaceous invertebrates were conducted. To determine the effect of GM soybeans, bacteriological studies of the colon of white outbred rats were carried out. The study studied 9 microorganisms from among the representatives of the colon microflora. Results: Analysis of the obtained results showed that the symptoms of colon dysbacteriosis were observed at the end of the observation period in laboratory animals that consumed GM soybeans. This situation is manifested in the following: the index of Bifidobacterium spp and Lactobacillus spp was significantly reduced by 2.43 and 3.05 times in animals that consumed GM soy compared to intact rats, which was interpreted as the first element of dysbiosis formed under the influence of GM soy. It has been proven that the germination of lactose-negative strains, the absence of lactose-positive strains is the second element of colon dysbiosis. In the main group, Enterobacter spp and Proteus spp increased by 4.54 and 3.75 times, respectively, compared with the control group, which turned out to be the third element of colon dysbiosis. if the representative of the indigenous microflora - nonpathogenic Streptococcus spp in the main group significantly decreased up to 1.47 times compared with intact laboratory animals, then the quantitative indicator of Staphylococcus spp significantly increased up to 1.50 times. This intergroup discrepancy has been interpreted as the fourth element of colonic dysbiosis. A significant increase in the quantitative index of Candida spp up to 1.94 times in 􀁔􀁛􀁡􀁟􀁘􀁗􀁛􀁩􀁛􀁠􀁓􀀃􀁕􀁓􀀃􀁓􀁟􀁓􀁞􀁛􀇜􀁥􀀃􀁙􀁦􀁣􀁠􀁓􀁞􀁛􀀃􀀃􀂬􀀃􀀃􀁙􀁦􀁣􀁠􀁓􀁞􀀃􀁔􀁛􀁡􀁟􀁘􀁗􀁛􀁩􀁛􀁠􀁮􀀃􀁛􀀃􀁢􀁣􀁓􀁝􀁥􀁛􀁝􀁛􀀃􀀃􀂬􀀃􀀃􀀉􀀎􀀔􀀑􀀍􀉮􀀋􀀃􀀎􀀅􀀃􀉸􀀈􀀎􀀌􀀄􀉖􀀈􀉹􀀈􀀍􀀄􀀃􀉮􀀍􀉖􀀃􀀏􀀑􀉮􀉹􀀓􀀈􀉹􀀄 №1 | 2023 67 outbred rats fed with GM soy, compared with those not fed with this product, is indicated as the fifth element of colon dysbiosis. GM soybeans have been shown to be the main factor causing this. Conclusions: In 2 out of 9 studied microorganisms (Staphylococcus spp, Candida spp), intergroup differences were not revealed, they were quantitatively close to each other. If all 5 of the listed elements of dysbiosis were present in laboratory animals that consumed GM soybeans, then they clearly did not appear in white outbred rats that consumed non-GM soybeans. In intact laboratory animals (group 1) there are no signs of dysbiosis, in animals fed non-GM soy (group 2) signs of dysbiosis are poorly developed (I degree of dysbiosis), and in those fed with GM soy, signs of dysbiosis are clearly expressed (II degree of dysbiosis). This situation was explained by the unfamiliarity of GM soybean in the organism of rats, the low resistance of both studied strains of microorganisms to environmental factors.

Key words

Keywords: GMO soy, white outbred rats, normal microflora, dysbiosis.

Literature

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